4Pi STED microscopy
= 4Pi STED microscopy
{wiki=4Pi_STED_microscopy}
4Pi STED microscopy is an advanced imaging technique in the field of fluorescence microscopy that enhances spatial resolution by combining two advanced methods: 4Pi microscopy and stimulated emission depletion (STED) microscopy. 1. **4Pi Microscopy**: This technique uses both the upper and lower hemispherical illumination of the sample, effectively capturing fluorescence from both sides.