= Nested polymerase chain reaction
{wiki=Nested_polymerase_chain_reaction}
Nested polymerase chain reaction (nested PCR) is a modification of the standard polymerase chain reaction (PCR) technique that is designed to increase the specificity and sensitivity of the amplification of a target DNA sequence. It involves two consecutive rounds of PCR using two sets of primers. \#\#\# Steps Involved in Nested PCR: 1. **First Round of PCR**: - The first set of primers (outer primers) is used to amplify a larger target region of the DNA.
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