= STED microscopy
{wiki=STED_microscopy}
STED microscopy, or Stimulated Emission Depletion microscopy, is a super-resolution imaging technique used in fluorescence microscopy. It was developed to overcome the diffraction limit of conventional light microscopy, which typically restricts resolution to about 200 nanometers for visible light. The fundamental principle behind STED microscopy involves the use of two laser beams: 1. **Excitation Beam**: This laser excites fluorescent molecules (fluorophores) in the sample, causing them to emit light.
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