EcoRV is a type of restriction enzyme, specifically a restriction endonuclease that is derived from the bacterium *Escherichia coli* (E. coli) strain RY13. It recognizes a specific DNA sequence and cleaves the double-stranded DNA at unique sites within that sequence. The recognition sequence for EcoRV is 5'–GAT^ATC–3', where the caret (^) indicates the position where the enzyme cuts the DNA.
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