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The main way to sequence <DNA methylation>. Converts methylated <cytosine> to <uracil>, and then we can sequence those.

\Video[https://www.youtube.com/watch?v=OcIazFGQv0g]
{title=Bisulfite Sequencing by Henrik's Lab (2020)}
{description=Nothing much new that we don't understand from a single sentence in the animation. But hey, animations!}


Bisulfite sequencing

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They are actually inheritable! But <alleles> are rare: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5559844

\Image[https://upload.wikimedia.org/wikipedia/commons/b/b3/Cloned_mice_with_different_DNA_methylation.png]
{title=To rats with the same genome differing only in <DNA methylation> with a different tail <phenotype>.}


Transgenerational epigenetic inheritance

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The first found and most important known <epigenetic> marker.

Happens only on <adenine> and <cytosine>. <Adenine methylation> is much less common in <mammal> than <cytosine> methylation, when people say "methylation" they often mean just cytosine methylation.

It often happens on <promoters>, where it inhibits <transcription>.


Ciro Santilli @cirosantilli 37

 Incoming links: DNA methylation