Equal opportunity

Chemical weapon

{wiki}

\Video[https://www.youtube.com/watch?v=EJR1H5tf5wE]
{title=One Million Dollars scene from Austin Powers}

\Video[https://www.youtube.com/watch?v=BRAkobf-tVI]
{title=100 Billion Dollars scene from Austin  Powers}


Inflation

For similar reasons as <Regulate the fuck out of advertising>{full}.
* https://petition.parliament.uk/archived/petitions/25994


Ban political donations

WordPress

Pieter Zeeman

Homology

GoDaddy

Physical Review Letters article

Protease

Carbon

{wiki}

* https://en.wikipedia.org/wiki/Causality
* https://en.wikipedia.org/wiki/Causality_(physics)


Causality

Pinyin

After filtration, all DNA should present in the filter, so we cut the paper up with scissors and put the pieces into an Eppendorf: <video Cutting vacuum pump filter and placing it in Eppendorf>{full}.

\Video[https://upload.wikimedia.org/wikipedia/commons/transcoded/8/85/Vacuum_pump_filter_cut_and_place_in_eppendorf.webm/Vacuum_pump_filter_cut_and_place_in_eppendorf.webm.480p.vp9.webm]
{height=400}
{title=Cutting vacuum pump filter and placing it in <Eppendorf>}

Now that we had the DNA in Eppendorfs, we were ready to continue the purification in a simpler and more standardized lab pipeline fashion.

First we added some small specialized beads and chemicals to the water and shook them Eppendorfs hard in a <Scientific Industries Inc. Vortex-Genie 2> machine to break the <cell (biology)> and free the DNA.

\Video[https://upload.wikimedia.org/wikipedia/commons/transcoded/e/e1/Scientific_Industries_Inc_Vortex-Genie_2_loading.webm/Scientific_Industries_Inc_Vortex-Genie_2_loading.webm.480p.vp9.webm]

\Video[https://upload.wikimedia.org/wikipedia/commons/transcoded/1/19/Scientific_Industries_Inc_Vortex-Genie_2_running.ogv/Scientific_Industries_Inc_Vortex-Genie_2_running.ogv.480p.vp9.webm]
{height=400}

Once that was done, we added several reagents which split the solution into two phases: one containing the DNA and the other not. We would then pipette the phase with the DNA out to the next Eppendorf, and continue the process.

In one step for example, the DNA was present as a white precipitate at the bottom of the tube, and we threw away the supernatant liquid: <image White precipitate formed with Qiagen DNeasy PowerWater Kit>{full}.

\Image[https://upload.wikimedia.org/wikipedia/commons/thumb/3/30/Qiagen_DNeasy_PowerWater_Kit_White_Precipitate.jpg/586px-Qiagen_DNeasy_PowerWater_Kit_White_Precipitate.jpg]
{title=White precipitate formed with Qiagen DNeasy PowerWater Kit}

At various stages, centrifuging was also necessary. Much like the previous vacuum pump step, this adds extra gravity to speed up the separation of phases with different molecular masses.

In our case, we used a <VWR Micro Star 17 microcentrifuge> for those steps:

\Image[https://upload.wikimedia.org/wikipedia/commons/thumb/0/03/VWR_Micro_Star_17_microcentrifuge.jpg/360px-VWR_Micro_Star_17_microcentrifuge.jpg]
{height=400}
{titleFromSrc}

\Image[https://upload.wikimedia.org/wikipedia/commons/thumb/6/65/VWR_Micro_Star_17_microcentrifuge_loading.png/358px-VWR_Micro_Star_17_microcentrifuge_loading.png]
{titleFromSrc}

Then, when we had finally finished all the purification steps, we measured the quantity of DNA with a <Biochrom SimpliNano spectrophotometer> to check that the purification went well:

\Image[https://upload.wikimedia.org/wikipedia/commons/thumb/4/47/Biochrom_SimpliNano_spectrophotometer_loading_sample.jpg/262px-Biochrom_SimpliNano_spectrophotometer_loading_sample.jpg]
{height=400}
{titleFromSrc}

\Image[https://upload.wikimedia.org/wikipedia/commons/thumb/f/f4/Biochrom_SimpliNano_spectrophotometer_result_readout.jpg/360px-Biochrom_SimpliNano_spectrophotometer_result_readout.jpg]
{titleFromSrc}

And because the readings were good, we put it in our -20 C fridge to preserve it until the second day of the workshop and called it a day:

\Image[https://upload.wikimedia.org/wikipedia/commons/thumb/f/f7/Minus_20_fridge_storing_samples.jpg/183px-Minus_20_fridge_storing_samples.jpg]
{height=400}
{titleFromSrc}


Post filtration purification

Nanometer

Python package manager

Digestive system

{disambiguate=video game}
{c}
{title2=1993}
{wiki=Doom_(franchise)}


Doom

The Pentagon

https://physics.stackexchange.com/questions/614757/intuitive-explanation-for-why-time-symmetry-implies-conservation-of-energy on <Physics Stack Exchange>


Title	Author	Created	Updated
Equal opportunity	Ciro Santilli 32	1970-01-01	1970-01-01
Chemical weapon	Ciro Santilli 32	1970-01-01	1970-01-01
Inflation	Ciro Santilli 32	1970-01-01	1970-01-01
Ban political donations	Ciro Santilli 32	1970-01-01	1970-01-01
WordPress	Ciro Santilli 32	1970-01-01	1970-01-01
Pieter Zeeman	Ciro Santilli 32	1970-01-01	1970-01-01
Homology	Ciro Santilli 32	1970-01-01	1970-01-01
GoDaddy	Ciro Santilli 32	1970-01-01	1970-01-01
Physical Review Letters article	Ciro Santilli 32	1970-01-01	1970-01-01
Protease	Ciro Santilli 32	1970-01-01	1970-01-01
Carbon	Ciro Santilli 32	1970-01-01	1970-01-01
Causality	Ciro Santilli 32	1970-01-01	1970-01-01
Pinyin	Ciro Santilli 32	1970-01-01	1970-01-01
Post filtration purification	Ciro Santilli 32	1970-01-01	1970-01-01
Nanometer	Ciro Santilli 32	1970-01-01	1970-01-01
Python package manager	Ciro Santilli 32	1970-01-01	1970-01-01
Digestive system	Ciro Santilli 32	1970-01-01	1970-01-01
Doom	Ciro Santilli 32	1970-01-01	1970-01-01
The Pentagon	Ciro Santilli 32	1970-01-01	1970-01-01
Time invariance implies energy conservation	Ciro Santilli 32	1970-01-01	1970-01-01

Ciro Santilli (@cirosantilli, 32)