 An Optimized Enzyme-Coupled Spectrophotometric Method for Measuring Pyruvate Kinase Kinetics by Saurabh Upadhyay

By Saurabh Upadhyay. Published in Bio-protocol 15(16), e5415, on 20 August 2025; available online 5 August 2025. Bio-protocol publishes and hosts the protocol; the author's Indian Institute of Technology, New Delhi affiliation is distinct from authorship. PMC is an archival mirror.

Online text: en.bio-protocol.org/en/bpdetail?id=5415&type=0

DOI: doi.org/10.21769/BioProtoc.5415

Cited passages: graphical overview caption; Equipment item 1; Procedure A.5–6 and B.2.a–i; Figure 2 caption; Data analysis §3; Validation of protocol; General notes items 4 and 6; PDF printed pp. 7–10. Procedure B.2.i and Data analysis §3 give different optical path lengths; no absolute reported rate is adopted.