 DNA sequencing with chain-terminating inhibitors by Frederick Sanger and others

By Frederick Sanger, S. Nicklen and Alan Coulson, printed as F. Sanger, S. Nicklen and A. R. Coulson. Their listed affiliation is the Medical Research Council Laboratory of Molecular Biology. Published in Proceedings of the National Academy of Sciences of the United States of America 74(12), 5463–5467, December 1977. The National Academy of Sciences is the publisher; PubMed Central hosts the archival record.

DOI: doi.org/10.1073/pnas.74.12.5463

Inspected full-text mirror of the same article: nemates.org/uky/425/Sanger.PNAS.1977.v74.p5463-5467.pdf

Online text: pmc.ncbi.nlm.nih.gov/articles/PMC431765/

Cited passages: PMC bibliographic record and abstract; Full paper PDF text at nemates.org/uky/425/Sanger.PNAS.1977.v74.p5463-5467.pdf; p. 5464, Sequencing Procedure: restriction-fragment primers, heating/annealing, Klenow polymerase, radioactive dATP and chase-omission observation; p. 5464, Figure 1 caption: G/A/T/C lanes, araCTP C reaction, 12% acrylamide, 40 mA, 14 h; p. 5465, Figure 2 caption: ddCTP/all-dideoxy comparison; p. 5466, Results: weak fast T bands, unresolved long runs, comparison with provisional sequence and structure-dependent migration.