A researcher whose team investigated how fluorescent microsphere size affects measurements of a confocal point spread function. A finite bead is an extended object, so its image includes both optical blur and its own dimensions. The study combines numerical calculations with measurements to assess that calibration bias.[ref]
Measurement of point-spread function (PSF) for confocal fluorescence microscopy by InCheon Song and others. Abstract and §§2–3: bead-size effects, numerical predictions and experimental comparison
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