 Quantitative proteomics

Quantitative proteomics measures differences in protein abundance or related protein features across samples. Strategies include label-free quantification and isotope- or tag-based comparisons, often using peptides generated from proteins and analyzed by mass spectrometry.

Peptide signal must be connected to the protein being quantified. Shared peptide sequences, incomplete detection, and changes in modification state can complicate that inference. Absolute abundance and relative change therefore require different calibration and interpretation.