 RNA-peptide fusions for the in vitro selection of peptides and proteins by Richard W. Roberts and Jack W. Szostak

Proceedings of the National Academy of Sciences USA 94(23), 12297–12302 (11 November 1997), DOI 10.1073/pnas.94.23.12297. Exact full first bylines Richard W Roberts and Jack W Szostak. Abstract and introductory rationale describe puromycin-mediated RNA-peptide fusions linking a peptide to its encoding mRNA; immunoprecipitation enriched an encoded myc epitope peptide from random fusion material.

Additional exact locator: Selective Enrichment of an RNA-Peptide Fusion and Figure 5, pmc.ncbi.nlm.nih.gov/articles/PMC24913/ . Before/after anti-myc immunoprecipitation aliquots were reverse-transcribed and PCR-amplified with a radiolabeled primer; restriction digestion distinguished the myc encoding sequence from the pool. The figure also notes preferential translation/reverse transcription, so recovered sequence ratios are not automatically unbiased input ratios.