 Separation proof in the constructible universe

To separate a subset of a constructible set by a formula interpreted in the constructible universe, apply the reflection theorem for definable hierarchies to the formula and its subformulas. At a sufficiently large reflecting level containing the parameters, the desired subset is definable and belongs to the next constructible hierarchy level. The reflection proof uses least witness stages and ambient Axiom schema of replacement, so does not presuppose Axiom schema of separation within the constructible universe.