Andrew Coffey Created 2026-10-14 Updated 2026-11-05
Chromatography separates components of a mixture through different migration rates in contact with a chromatographic stationary phase while a chromatographic mobile phase transports them. Liquid chromatography describes the physical state of the transporting phase; size-exclusion chromatography describes a separation mechanism. Classification by phase and classification by mechanism answer different questions. In ideal size-exclusion chromatography, differential access to liquid-filled pores replaces binding as the source of differential migration.
By Andrew Coffey.
Publisher: Agilent Technologies UK Ltd..
Published in HPLC 2013, poster 901.
Online text: www.agilent.com/Library/posters/Public/2013_HPLC_Andrew_Coffey_SEC_Detector_combinations.pdf
Cited passages: Andrew Coffey, Size Exclusion Chromatography of Biomolecules – Detector Combinations to Obtain Maximum Information with Minimum Effort, HPLC 2013, poster 901: Experimental; Figures 3–7; Table 1.