= Auxin response factor 1
\b[Auxin response factor 1], historically abbreviated ARF1 in this plant context, is a <transcription factor> characterized for its ability to bind <auxin response elements>. The 1997 report isolated it using a <yeast one-hybrid assay> and related its DNA-binding specificity to sequences that conferred an <auxin>-responsive transcriptional effect.<ARF1, a transcription factor that binds to auxin response elements by T. Ulmasov and others>[Abstract, Science276 (1997), pp.1865–1868; DOI10.1126/science.276.5320.1865.]{ref}
The study identified an amino-terminal <DNA-binding domain> and a distinct carboxyl-terminal region associated with interactions between <proteins>. This supplies a concrete example of modular regulation: contacting a target DNA sequence and contacting another regulator can involve different parts of one <protein>.<ARF1, a transcription factor that binds to auxin response elements by T. Ulmasov and others>[Abstract: amino-terminal DNA recognition and carboxyl-terminal interaction region.]{ref}
Experiments with <DR5 auxin response elements> also showed ARF1 binding and interactions with <Aux IAA proteins>. Those observations connect a named factor to a defined regulatory system. They do not turn every <auxin response factor> into the same protein, nor make DNA binding alone a complete account of the resulting <gene expression>.<Aux IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements by T. Ulmasov and others>[Abstract: ARF1 binding, interaction and reporter repression comparisons.]{ref}
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