= Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase by MilliporeSigma
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https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-alkaline-phosphatase-diethanolamine-assay
Manufacturer primary method, §4 reaction and §§7.4–7.5 spectrophotometric measurement/calculations:405nm, blank subtraction and millimolar extinction coefficient18.5. §7.1 displays750nm, inconsistent with the repeated405nm statements in test/calculation sections; article uses the latter and no preparation recipe.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/116/490/p0405enz.pdf
Sigma P0405 original assay document: p.1, assay principle and conditions; p.2, 405nm response coefficient under the stated conditions.
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