Manufacturer primary method, §4 reaction and §§7.4–7.5 spectrophotometric measurement/calculations:405nm, blank subtraction and millimolar extinction coefficient18.5. §7.1 displays750nm, inconsistent with the repeated405nm statements in test/calculation sections; article uses the latter and no preparation recipe.
Sigma P0405 original assay document: p.1, assay principle and conditions; p.2, 405nm response coefficient under the stated conditions.

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