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The Dirac field has the global symmetry $\psi \mapsto e^{i\alpha }\psi$, $\bar{\psi }\mapsto e^{-i\alpha }\bar{\psi }$, while the real scalar is unchanged. Both the free Dirac action and the pseudoscalar Yukawa interaction preserve this symmetry. Therefore Dirac fermion number conservation holds: its charge counts particles minus antiparticles.

The initial state has charge $+1$, whereas a final antiparticle and a neutral scalar have charge $-1$. Since the S-matrix commutes with that charge,This holds at every order, not just tree level. In the Feynman rules, a continuous fermion arrow cannot connect these specified external states. Replacing only the outgoing $\bar{u}$ by a $v$ in the previous expression would not give a physical amplitude. Changing both external fermions to antiparticles would instead produce an allowed process with reversed fermion flow and the appropriate $v$ spinors; moving a leg between initial and final states is a different operation governed by crossing symmetry.

A pseudoscalar Yukawa interaction couples a Dirac field bilinear containing the chirality matrix to a spin-zero field. In the standard mostly-minus gamma matrix convention with ${\gamma }^5=i{\gamma }^0{\gamma }^1{\gamma }^2{\gamma }^3$ and the usual Dirac adjoint, $ig\bar{\psi }{\gamma }^5\psi \varphi$ is Hermitian for real $g$ and real $\varphi$. If $\varphi$ is a pseudoscalar, the interaction also preserves parity symmetry in quantum field theory; if $\varphi$ is parity even, it breaks that symmetry. Moving the factor $i$ into the definition of ${\gamma }^5$ changes the displayed vertex coefficient but not the interaction. Each vertex has one bosonic leg and two oppositely oriented fermion legs, and preserves Dirac fermion number conservation.