Nanopore sequencing Created 2026-10-20 Updated 2026-11-05
Nanopore sequencing infers a nucleic-acid sequence from changes in electrical current as a strand passes through a nanometre-scale pore. The signal depends on several nearby bases rather than one perfectly isolated base at a time. It supports long reads and direct RNA measurement, but base calling and modification inference require calibrated models. Comparison of conditions can detect altered signal patterns without identifying every chemical modification uniquely. [ref]
Identification of differential RNA modifications from nanopore direct RNA sequencing with xPore by Ploy N. Pratanwanich and others. 2021, current distributions, differential modification inference and direct RNA data
Ploy N. Pratanwanich Created 2026-10-20 Updated 2026-11-05
Coauthor of the xPore study identifying differential RNA modifications from direct nanopore sequencing. The method connected changes in measurement signals with comparisons of modification patterns between conditions.[ref]
xPore 2026-10-20
A method for detecting differential RNA-modification signals using Gaussian mixture distributions of nanopore current, with condition-specific component proportions. A shifted signal requires biological interpretation as well as statistical separation.[ref]
Identification of differential RNA modifications from nanopore direct RNA sequencing with xPore by Ploy N. Pratanwanich and others. Method overview: shared current distributions and sample-specific modification fractions