Nanopore sequencing infers a nucleic-acid sequence from changes in electrical current as a strand passes through a nanometre-scale pore. The signal depends on several nearby bases rather than one perfectly isolated base at a time. It supports long reads and direct RNA measurement, but base calling and modification inference require calibrated models. Comparison of conditions can detect altered signal patterns without identifying every chemical modification uniquely. [ref]
Identification of differential RNA modifications from nanopore direct RNA sequencing with xPore by Ploy N. Pratanwanich and others. 2021, current distributions, differential modification inference and direct RNA data
A method for detecting differential RNA-modification signals using Gaussian mixture distributions of nanopore current, with condition-specific component proportions. A shifted signal requires biological interpretation as well as statistical separation.[ref]
Identification of differential RNA modifications from nanopore direct RNA sequencing with xPore by Ploy N. Pratanwanich and others. Method overview: shared current distributions and sample-specific modification fractions
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Nanopore sequencing is a next-generation sequencing technology that allows for the real-time analysis of nucleic acids, such as DNA and RNA, by passing them through small nanopores—tiny openings that measure just a few nanometers in diameter.