= J. Choay
{c}
<J. Choay> is the first author of a 1983 study testing which defined fragments of <heparin> support binding to <antithrombin>. The team synthesized related four- and five-sugar structures, checked their chemical structures by <nuclear magnetic resonance>, and measured binding and enhancement of <factor Xa> inhibition.<Structure-activity relationship in heparin: a synthetic pentasaccharide with high affinity for antithrombin III and eliciting high anti-factor Xa activity by J. Choay and others>[Abstract; Biochemical and Biophysical Research Communications 116, 492–499, 1983]{ref}
The tested five-sugar fragment formed an equimolar complex with <antithrombin> and enhanced its inhibitory activity; the four-sugar fragment did not show the same behavior. Comparing chemically defined fragments helped separate a particular residue arrangement from the heterogeneous mixture of chains in a <heparin> preparation. Structure confirmation and activity measurement answered different questions, and both were needed for the argument.<Structure-activity relationship in heparin: a synthetic pentasaccharide with high affinity for antithrombin III and eliciting high anti-factor Xa activity by J. Choay and others>[Abstract: synthetic tetrasaccharide/pentasaccharide comparison, binding and anti-factor Xa activity]{ref}
Back to article page
