= J. G. Stavrianopoulos
{disambiguate=RNase H researcher}
{c}
<J. G. Stavrianopoulos (RNase H researcher)> is the first author of a 1973 study purifying and characterizing calf-thymus <Ribonuclease H>. The investigators enriched the activity more than 3000-fold, then tested the preparation on defined <RNA-DNA hybrids> and on other <RNA> material. Activity required suitable metal ions, including <magnesium> or <manganese> under the reported conditions.<Purification and properties of ribonuclease H of calf thymus by J. G. Stavrianopoulos and others>[Abstract: purification, activating ions and substrate tests; PNAS 70, 1959–1963, 1973]{ref}
The preparation cleaved the tested <RNA-DNA hybrids> but did not attack <ribosomal RNA>. A negative substrate result is informative here: the name ribonuclease alone does not specify which molecular arrangement an <enzyme> recognizes. Comparing paired nucleic-acid substrates with an RNA preparation lacking the DNA partner helped characterize specificity rather than merely establishing that some cleavage occurred.<Purification and properties of ribonuclease H of calf thymus by J. G. Stavrianopoulos and others>[Abstract: synthetic hybrid, phage-DNA/RNA hybrid and ribosomal-RNA negative result]{ref}
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