J. G. Stavrianopoulos is the first author of a 1973 study purifying and characterizing calf-thymus Ribonuclease H. The investigators enriched the activity more than 3000-fold, then tested the preparation on defined RNA-DNA hybrids and on other RNA material. Activity required suitable metal ions, including magnesium or manganese under the reported conditions.[ref]
Purification and properties of ribonuclease H of calf thymus by J. G. Stavrianopoulos and others. Abstract: purification, activating ions and substrate tests; PNAS 70, 1959–1963, 1973
The preparation cleaved the tested RNA-DNA hybrids but did not attack ribosomal RNA. A negative substrate result is informative here: the name ribonuclease alone does not specify which molecular arrangement an enzyme recognizes. Comparing paired nucleic-acid substrates with an RNA preparation lacking the DNA partner helped characterize specificity rather than merely establishing that some cleavage occurred.[ref]
Purification and properties of ribonuclease H of calf thymus by J. G. Stavrianopoulos and others. Abstract: synthetic hybrid, phage-DNA/RNA hybrid and ribosomal-RNA negative result
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