= M. Desjardins
{c}
M. Desjardins is a coauthor of a 1994 experimental study of how a <phagosome> becomes a <phagolysosome>. The researchers followed <phagosomes> containing latex beads in <macrophages>, isolating these compartments by <density-gradient centrifugation> and measuring changes in their <proteins>. The beads supplied a physical handle for recovering compartments formed around ingested material.<Biogenesis of phagolysosomes proceeds through a sequential series of interactions with the endocytic apparatus by M. Desjardins and others>[Abstract and Materials and methods: bead-containing phagosome preparation.]{ref}
The study found successive exchanges with the <endocytosis> system rather than a single instantaneous conversion. Changes in membrane markers, together with microscopy, made <phagosome> maturation observable as a process. An illuminating comparison is to follow the same class of bead-containing <phagosomes> shortly after ingestion and later: both contain beads, but their <protein> composition differs. Thus counting engulfed particles measures one part of <phagocytosis>, while examining their surrounding compartments reveals subsequent maturation.<Biogenesis of phagolysosomes proceeds through a sequential series of interactions with the endocytic apparatus by M. Desjardins and others>[Results, Figures 5–7: acquisition of endocytic markers and time-dependent maturation.]{ref}
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