M. Desjardins is a coauthor of a 1994 experimental study of how a phagosome becomes a phagolysosome. The researchers followed phagosomes containing latex beads in macrophages, isolating these compartments by density-gradient centrifugation and measuring changes in their proteins. The beads supplied a physical handle for recovering compartments formed around ingested material.[ref]
Biogenesis of phagolysosomes proceeds through a sequential series of interactions with the endocytic apparatus by M. Desjardins and others. Abstract and Materials and methods: bead-containing phagosome preparation.
The study found successive exchanges with the endocytosis system rather than a single instantaneous conversion. Changes in membrane markers, together with microscopy, made phagosome maturation observable as a process. An illuminating comparison is to follow the same class of bead-containing phagosomes shortly after ingestion and later: both contain beads, but their protein composition differs. Thus counting engulfed particles measures one part of phagocytosis, while examining their surrounding compartments reveals subsequent maturation.[ref]
Biogenesis of phagolysosomes proceeds through a sequential series of interactions with the endocytic apparatus by M. Desjardins and others. Results, Figures 5–7: acquisition of endocytic markers and time-dependent maturation.
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