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The printed assertion for every cohomological degree is false. The valid conclusion supplied by the stated Hartogs extension theorem is the degree-zero isomorphism. Indeed, around each removed point choose a small coordinate ball on which the holomorphic vector bundle $E$ is trivial. A holomorphic section on the punctured ball has finitely many holomorphic coefficient functions, each of which extends by Hartogs extension theorem when the complex dimension is at least two. Uniqueness of holomorphic extension makes these local extensions agree with the original section on overlaps, givingThis also identifies the direct image sheaf $j_{\ast }\mathcal{O}(E{|}_{X_0})$ with $\mathcal{O}(E)$ for the inclusion $j:X_0\hookrightarrow X$. It does not identify higher sheaf cohomology.

Here is an explicit higher-degree obstruction. Take $X={\mathbb{P}}^2$, remove $[0:0:1]$, and take the trivial line bundle. Cover $X_0$ by $U=\{x\ne 0\}$ and $V=\{y\ne 0\}$. On $U$ use coordinates $t=y/x$, $s=z/x$; on $V$ use $u=x/y=1/t$, $v=z/y=s/t$. Both charts are ${\mathbb{C}}^2$, and the overlap is ${\mathbb{C}}^{\ast }\times \mathbb{C}$. For every integer $m\ge 2$, the holomorphic functiondefines a Čech cocycle. It cannot be a Čech coboundary $b(1/t,s/t)-a(t,s)$ with $a,b$ entire on their charts. Taking the coefficient of $s^m$, the term from $a$ has only nonnegative powers of $t$, whereas the term from $b$ has the form $t^{-m}{b}_m(1/t)$ and only powers at most $-m$. Neither can provide the coefficient $t^{-1}$. Uniqueness of Laurent series proves the contradiction. The same argument applied to each fibre degree proves that all the classes $[g_m]$, $m\ge 2$, are linearly independent.

These classes remain nonzero in Čech cohomology of $X_0$, rather than merely of this cover. The low-degree Mayer-Vietoris sequence for sheaf cohomology injects the quotient of overlap sections by the two chart-section groups into $H^1(X_0,\mathcal{O})$. Concretely, a partition of unity gives smooth $f_U,f_V$ with $f_V-f_U=g$; their common Dolbeault operator defines a global $(0,1)$-form. If that form were $\bar{\partial }$-exact, subtracting its global smooth primitive would make $f_U,f_V$ holomorphic and split $g$. The nonsplitting just proved therefore gives the same obstruction in Dolbeault cohomology, and the Dolbeault theorem identifies it with sheaf cohomology. Thus $H^1(X_0,\mathcal{O})$ is infinite dimensional. In contrast, $H^1({\mathbb{P}}^2,\mathcal{O})$ is finite dimensional by compact Dolbeault Hodge decomposition for the Fubini-Study form. Hence the two degree-one groups cannot be isomorphic, disproving the printed all-degree request already in complex dimension two.

In complex dimension one, even the degree-zero conclusion fails. Take $X={\mathbb{P}}^1$, remove its point at infinity and use the trivial line bundle. Holomorphic functions on compact connected ${\mathbb{P}}^1$ are constant by the maximum modulus principle, whereas $X_0\cong \mathbb{C}$ has nonconstant entire functions such as $z$. Therefore the restriction map is not onto even in degree zero.

For $X=U\cup V$, the Mayer-Vietoris sequence for sheaf cohomology is the long exact sequence

We prove the required vanishing by induction on the number $m$ of open sets. The case $m=1$ is an assumption. Put $U=U_1\cup \cdots \cup U_{m-1}$ and $V=U_m$. The induction hypothesis gives $H^p(U,\mathcal{F})=0$ for every $p$. The intersections $U_i\cap U_m$ cover $U\cap V$, and every nonempty finite intersection among them is one of the intersections in the hypothesis, so the same induction gives $H^p(U\cap V,\mathcal{F})=0$. We also have $H^p(V,\mathcal{F})=0$. Exactness of the Mayer-Vietoris sequence now yields

For the cover $\mathcal{U}=\{X_1,X_2\}$ of the affine plane with doubled origin, the overlap is the punctured affine plane $U$. Since $H^0(U,{\mathcal{O}}_U)=k[x,y]$, the Čech complex beginsThis map is surjective, and the normalized complex has no terms in degrees at least two. Consequently

The Mayer-Vietoris sequence for sheaf cohomology also gives $H^1(Y,{\mathcal{O}}_Y)=0$, but its next part givesPart b with $f=1$ shows that the group on the right is infinite-dimensional. Thus

This does not contradict the acyclic cover theorem. Although $X_1$ and $X_2$ are affine, their intersection $U$ is not acyclic: it has nonzero first structure-sheaf cohomology. Equivalently, this affine cover does not satisfy the theorem's hypotheses; the doubled-origin plane is not a semi-separated scheme.