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Reflection through the projection plane reverses the orientation of $S^3$ and exchanges the overpassing and underpassing strands at every crossing. Thus switching all crossings gives the mirror of a link, with the component orientations transported by the reflection.

Define $W(L)(q)=V(\overline{L})(q^{-1})$. Reflection exchanges $D_{-}$ with $D_{+}$ and preserves the oriented smoothing. Apply the printed skein relation to the reflected triple at parameter $q^{-1}$:Multiplication by $-1$ gives the original skein relation for $W$. Also $W(U_1)=1$. The stated uniqueness of the Jones polynomial now gives $W=V$, hence

Let $H_{+}$ be the positively oriented Hopf link obtained by closing a two-strand braid with two positive crossings. Switching one crossing gives the two-component unlink; the oriented smoothing gives the unknot. The printed skein relation givesRepresent the right-handed trefoil knot by the closure of the two-strand braid with three positive crossings. Switching one crossing gives the unknot, while its oriented smoothing gives $H_{+}$. Consequentlyso the two Jones polynomials areThe second expression follows from the mirror of a link identity. They are unequal Laurent polynomials, so invariance under link isotopy proves that the right-handed and left-handed trefoils are not isotopic.