Nicholas J. Severs
= Nicholas J. Severs
{c}
<Nicholas J. Severs> is a researcher in cellular ultrastructure whose 2007 <freeze-fracture electron microscopy> protocol lists an affiliation with the National Heart and Lung Institute at Imperial College London. His methodological work explains how fracture planes reveal the internal organization of <cell membranes>.<Freeze-fracture electron microscopy by Nicholas J. Severs>[Author affiliation and abstract, Nature Protocols 2, pp.547–576]{ref}
The protocol freezes a biological specimen, fractures it, deposits <platinum> and <carbon> to form a replica, and cleans that replica for <transmission electron microscopy>. The microscope thus examines a durable record of the exposed surface. Optional removal of ice by sublimation exposes additional relief. This makes preparation a scientific part of the measurement: the observed surface depends on where the specimen fractured and which material the procedure exposed.<Freeze-fracture electron microscopy by Nicholas J. Severs>[Abstract, four preparation stages, etching and planar membrane views]{ref}