Use the homotopy lifting theorem for a covering map: a homotopy of paths has a unique lift once its initial path is lifted. Also use uniqueness of lifts to a covering space: a lift of a constant path with a specified starting point is constant.
Let be a loop based at whose image under is trivial. There is a homotopy from to the constant loop at , fixing both endpoints. Lift this homotopy with initial path . Both vertical boundary paths are constant lifts at . Its final path is a lift of the constant loop and hence is also constant at . Thus the lifted homotopy contracts relative to its endpoints. The kernel of the induced group homomorphism is trivial, provingNo universal-cover assumption is needed for this injection; it holds for any actual covering map.
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