Solution (source code)

= Solution

Continue in the bistable regime $k>2$. At $s=0$ the reaction function factors as $f(g,0)=g(kg-1-g^2)/(1+g^2)$. Besides $g=0$, its positive equilibria are
$$
\boxed{g_1=\frac{k-\sqrt{k^2-4}}2<1,\qquad g_2=\frac{k+\sqrt{k^2-4}}2>1.}
$$
At zero, $f_g=-1$, so it is stable. At a positive equilibrium, use $k=(1+g^2)/g$ to obtain $f_g=(1-g^2)/(1+g^2)$. Therefore $g_1$ is unstable and $g_2$ stable. The sign of $f$ is negative on $(0,g_1)$, positive on $(g_1,g_2)$ and negative beyond $g_2$; these signs determine the requested reaction-rate sketch.

The stationary points of $f(g,0)$ obey $(1+g^2)^2=2kg$. For $k>2$ its smaller solution $g_c<1$ is the local minimum, and its larger solution lies above one and is the local maximum. Since increasing $s$ translates the whole graph upward, the low and middle equilibria collide when
$$
\boxed{s_c=-f(g_c,0)=\frac{g_c(1-g_c^2)}2,\qquad (1+g_c^2)^2=2kg_c.}
$$
For $s>s_c$, both stationary values of $f$ are positive, while $f(g,s)\to-\infty$ as $g\to\infty$; there is exactly one nonnegative equilibrium, on the descending high branch. At equality a double low equilibrium remains, so the uniqueness assertion requires strict exceedance.

For completeness, if the later positive-input request is considered for arbitrary $k>0$, the derivative is $f_g=2kg/(1+g^2)^2-1$. Its maximum is $9k/(8\sqrt3)-1$, attained at $g=1/\sqrt3$. For $k\leq8\sqrt3/9$, the reaction graph is decreasing and every $s>0$ already has a unique steady state, so take $s_c=0$. For $k>8\sqrt3/9$, the smaller positive stationary point gives the same $s_c=g_c(1-g_c^2)/2$; above it the graph crosses zero only on its final descending branch. Persistent robust switching after input is removed uses the previously assumed bistable regime $k>2$.

Starting near zero, raise $s$ past $s_c$ and keep it there long enough for $g$ to pass the unstable threshold and approach the high branch. Decrease $s$ back to zero slowly, or after ensuring $g>g_1$. The trajectory then stays in the basin of the high <stable equilibrium> and ends at the displayed $g_2$. \b[The persistent switched state is $g_2=(k+\sqrt{k^2-4})/2$.] This is the <saturating autocatalytic switch>; the conclusion presupposes $k>2$ and enough forcing duration, not merely an arbitrarily short pulse of large amplitude.

\Image[/past-exam-of-the-mathematics-course-of-the-university-of-cambridge/2008/ii/paper-1-gene-switch.png]
{title=Feedback production and degradation curves showing bistability and a sustained switching pulse}