Inclusion and passage to the quotient give a short exact sequence of chain complexes
Define the connecting homomorphism as follows. If , then , so . Since , it is a chain cycle in , and we set
Changing by a relative boundary or a chain in changes by a boundary in , so is well defined.
The resulting sequence is
We verify exactness directly. If a cycle of maps to zero in the relative homology, it differs from a boundary by a cycle in , so it lies in . If a relative cycle satisfies , then for some ; hence is a cycle in mapping to . Finally, if a cycle becomes a boundary in , say , then is a relative cycle with . The reverse containments follow immediately from consecutive composites being zero. This proves the long exact sequence in relative homology.

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