Point accumulation for imaging in nanoscale topography
= Point accumulation for imaging in nanoscale topography
= PAINT
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{synonym}
Transiently binding fluorescent probes provide spatially separated single-emitter observations whose fitted positions accumulate into a structural map. Probe exchange can replenish bleached emitters. Precision, specificity, event density, optical background and drift all affect the usable resolution; accumulating events does not itself remove systematic labelling error.