= SEC total accessible liquid volume
{c}
{title2=$V_t=V_0+V_i$}
The <SEC total accessible liquid volume> is $V_t=V_0+V_i$: interparticle plus pore liquid. A fully included nonbinding tracer estimates it. Geometric bed volume instead includes solid matrix, $V_c=V_t+V_{\rm matrix}$.<Size Exclusion Chromatography: Principles and Methods by GE Healthcare>[GE Healthcare, Size Exclusion Chromatography: Principles and Methods, revision 18102218 AM, printed pp. 15–16, Figure 1.7: liquid, pore and geometric volumes]{ref}
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