For the three-region calcium nullcline, defineFor , , as , and . Thus there is exactly one positive root for every positive . The curve starts at the origin and initially satisfies .
On the large- branch away from the crossing, replace by one. This gives where this is large and positive. Near retain the next term and linearize :Finally where the leak is negligible compared with the other terms, , so on the side . These are overlapping asymptotic descriptions with different validity ranges, not three exact disconnected curves. The curve rises to a large peak, falls sharply near , then approaches a small finite level as . At leading small- order that last level is .
