= Western blot controls
<Western blot loading controls> ask how much sample entered each lane; <Western blot transfer controls> ask what reached the receiving sheet; <Western blot antibody specificity controls> ask what the <antibodies> recognized. Each closes a different gap in <Western blot band interpretation>.
\Image[/western-blot-lane-control-interpretation.png]
{title=Conceptual lanes for <Western blot controls>}
{description=Original schematic, with invented qualitative marks only. Candidate bands disappear in a target-deleted sample while a lower band persists; a separate panel shows how a loading or transfer difference can accompany a target-band difference. No lane, darkness, band position or relative amount represents a measurement, a specific real <protein> or confirmed identity. The no-primary comparison denotes a matched strip processed without <primary antibody>.}
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