The first thing we did was to filter the water samples with a membrane filter that is so fine that not even bacteria can pass through, but water can.

Therefore, after filtration, we would have all particles such as bacteria and larger dirt pieces in the filter.

From the 1 liter in each bottle, we only used 400 ml because previous experiments showed that filtering the remaining 600 ml is very time consuming because the membrane filter gets clogged up.

Therefore, the filtration step allows us to reduce those 400 ml volumes to more manageable <Eppendorf tube> volumes: <image An Eppendorf tube>{full}. Reagents are expensive, and lab bench centrifuges are small!

\Image[https://upload.wikimedia.org/wikipedia/commons/thumb/3/3f/Microcentrifuge_tube_in_hand.jpg/640px-Microcentrifuge_tube_in_hand.jpg]
{title=An <Eppendorf tube>}
{description=They are small, convenient and disposable.}

\Image[https://upload.wikimedia.org/wikipedia/commons/thumb/3/35/Labelled_Eppendorf_microcentrifuge_tubes_on_rack.jpg/640px-Labelled_Eppendorf_microcentrifuge_tubes_on_rack.jpg]
{title=Labelled Eppendorf tubes on a rack}

Since the filter is so fine, filtering by gravity alone would take forever, and so we used a vacuum pump to speed thing up!

For that we used:
* <Thermo Scientific Nalgene Polysulfone Reusable Bottle Top Filters>
* <KNF Laboport series laboratory vacuum pump>

\Image[https://upload.wikimedia.org/wikipedia/commons/6/6e/Vacuum_pump_filter_peel_filter.png]
{title=Peeling the vacuum pump filter protection peel before usage}
{height=400}

\Image[https://upload.wikimedia.org/wikipedia/commons/7/78/Vacuum_pump_filter_place_filter.png]
{title=Placing the vacuum pump filter}

\Video[https://upload.wikimedia.org/wikipedia/commons/transcoded/3/3f/Vacuum_pump_filter_pour_sample_and_turn_on.webm/Vacuum_pump_filter_pour_sample_and_turn_on.webm.480p.vp9.webm]
{title=Pouring the water sample into the vacuum tube and turning on the vacuum pump}
{height=400}


Oxford nanopore river bacteria/filtration with vacuum pump

ID: oxford-nanopore-river-bacteria/filtration-with-vacuum-pump