Pulsed-field gel electrophoresis

ID: pulsed-field-gel-electrophoresis

Pulsed-field gel electrophoresis (PFGE) is a laboratory technique used to separate large DNA molecules by applying an electric field that periodically changes direction. This method is particularly effective for the analysis of large fragments of DNA, generally ranging from about 20 kilobases (kb) to several megabases, which are too large to be effectively separated by standard gel electrophoresis techniques.

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