Spectrophotometry is an analytical method used to measure the intensity of light absorbed by a sample at specific wavelengths. This technique is based on the principle that substances absorb light at characteristic wavelengths, and the amount of light absorbed can be related to the concentration of the substance in the sample according to Beer-Lambert's Law. ### Key Concepts: 1. **Light Absorption**: When light passes through a sample, certain wavelengths are absorbed while others may be transmitted or reflected.
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