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Ciro Santilli
@cirosantilli
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How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
/
Qiagen DNeasy PowerWater Kit
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How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it
/
DNA extraction
Updated
2025-04-24
+
Created
1970-01-01
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The
first
thing
we
had to do with the sample was to extract the
DNA
present
in the
water
in
a
pure form for the PCR.
We
did that with
a
Qiagen DNeasy PowerWater Kit
.
As
you would
expect
, this consists of
a
purification procedure with several steps.
In each step
we
take
a
physical or chemical action on the sample, which splits it into two parts: the one with the
DNA
and the one without.
We
then take the part with the
DNA
, and throw away the one without the
DNA
.
The
first
steps are coarser, and finer and finer splits are
done
as
we
move forward.
Total
articles
:
1