De novo transcriptome assembly

De novo transcriptome assembly is the process of reconstructing the complete set of RNA transcripts in a given organism or sample without prior reference to a known genome. This is particularly useful in situations where the genome of the organism is not available, poorly annotated, or when studying non-model organisms. Here are the key steps and concepts involved in de novo transcriptome assembly: 1. **RNA Extraction**: First, RNA is extracted from the cells or tissues of interest.