Edman degradation

Edman degradation is a well-established method for the sequential identification of amino acids in a peptide or protein. This technique allows for the determination of the amino acid sequence from the N-terminus of a peptide. Here is a brief overview of the process: 1. **Chemical Reaction**: The method involves the use of phenylisothiocyanate (PITC), which reacts with the free amino group of the N-terminal amino acid of the peptide.