Fluorescence Cross-Correlation Spectroscopy (FCCS) is a sophisticated optical technique used to study molecular interactions and dynamics at the single-molecule level. It combines principles from fluorescence spectroscopy and correlation methods to provide insights into the behavior of fluorescently labeled molecules in a solution. ### Key Concepts of FCCS: 1. **Fluorescence**: FCCS involves the use of fluorescent probes that emit light upon excitation. These probes can be attached to different molecules of interest.
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