Fluorescence spectroscopy is an analytical technique used to measure the fluorescence emitted by a substance after it has absorbed light or other electromagnetic radiation. This technique is based on the principle of fluorescence, where certain molecules (fluorophores) absorb light at a specific wavelength and subsequently emit light at a longer wavelength.
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Spectroscopy measuring emitted light following excitation. Emission peak position and intensity can report changes of speciation, but concentration, excitation absorption and photochemical loss also influence the signal.