GUIDE-Seq

GUIDE-Seq (Genome-wide Unbiased Identification of DNGs Enabled by Sequencing) is a technique used for the detection and identification of double-strand breaks (DSBs) in the genome caused by targeted genome editing methods, such as CRISPR/Cas9. The method is particularly useful for mapping the sites of DSBs generated by CRISPR, allowing researchers to evaluate the specificity and efficiency of gene editing events.