High-resolution melting analysis

High-resolution melting (HRM) analysis is a molecular technique used to analyze and quantify variations in DNA sequences. It leverages the principles of DNA melting and fluorescence to provide real-time insight into the melting behavior of DNA amplicons (PCR products) as they are gradually heated. Here’s a brief overview of how it works and its applications: ### Principles of HRM: 1. **DNA Melting**: DNA strands are held together by hydrogen bonds between complementary bases.