Christian de Duve investigated intracellular organization using biochemical fractionation. Work with Jacques Berthet examined a latent form of acid phosphatase, connecting enzyme accessibility with its subcellular association.
An enzyme can be present but poorly detected if a membrane limits substrate access. Comparing activity before and after disruption therefore helps distinguish low enzyme abundance from biochemical latency.[ref]
Tissue fractionation studies. 1. The existence of a mitochondria-linked, enzymically inactive form of acid phosphatase in rat-liver tissue by Jacques Berthet and Christian de Duve. 1951, page 174: rationale and fractionation
A biochemist.[ref]
https://www.rockefeller.edu/our-scientists/christian-de-duve/2448-nobel-prize/. Rockefeller University, Nobel Prize in Physiology or Medicine: identifies Christian de Duve as a Belgian biochemist
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