 Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase by MilliporeSigma

Manufacturer primary method, §4 reaction and §§7.4–7.5 spectrophotometric measurement/calculations:405nm, blank subtraction and millimolar extinction coefficient18.5. §7.1 displays750nm, inconsistent with the repeated405nm statements in test/calculation sections; article uses the latter and no preparation recipe.

Sigma P0405 original assay document: p.1, assay principle and conditions; p.2, 405nm response coefficient under the stated conditions.