The FLAG tag is an engineered epitope tag with the eight-residue sequence DYKDDDDK. Tag-specific antibodies permit detection or affinity capture of a fused protein. Its short length makes it convenient but does not guarantee neutrality: placement near a targeting sequence or functional interface can change behaviour. Comparing the tagged construct with an appropriate untagged or independently validated version tests whether it preserves the relevant function. [ref]
A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification by T. P. Hopp and others. Hopp and others, 1988, A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification
The FLAG-tag is a short peptide sequence that is commonly used in molecular biology for the purification and detection of proteins. The FLAG peptide consists of eight amino acids, typically represented as DYKDDDDK. It was developed to facilitate the identification and isolation of proteins within cells and various biological systems.
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