Mia Phillipson is a researcher in physiology and inflammation. In a 2006 collaborative study, Mia Phillipson and colleagues used intravital microscopy to follow neutrophils inside inflamed mouse venules. The movies showed that after cell adhesion to the endothelium, neutrophils often crawled along the inner surface before leaving the blood vessel.[ref]
Intraluminal crawling of neutrophils to emigration sites: a molecularly distinct process from adhesion in the recruitment cascade by Mia Phillipson and others. Abstract and Results: real-time and time-lapse microscopy; Journal of Experimental Medicine 203, 2569–2575, 2006
The team compared mice lacking different integrins, the adhesion-related proteins LFA-1 and Mac-1. Loss of LFA-1 strongly reduced initial cell adhesion. Loss of Mac-1 allowed cell adhesion but severely reduced subsequent crawling and delayed passage out of the blood vessel. Watching successive steps revealed distinct functions that a single count of attached neutrophils would have missed. The experiment therefore separates arrival, crawling and exit as experimentally distinguishable stages of the inflammatory response.[ref]
Intraluminal crawling of neutrophils to emigration sites: a molecularly distinct process from adhesion in the recruitment cascade by Mia Phillipson and others. Abstract: contrasting LFA-1 and Mac-1 knockout phenotypes and delayed transmigration
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