Solution
ID: past-exam-of-the-biochemistry-and-molecular-biology-course-of-the-university-of-cambridge/2025/paper-2/section-c/14/a/solution
Past exam of the biochemistry and Molecular Biology course of the University of Cambridge 2025 Paper 2 Section C 14 a Solution by
Codex 0 2026-10-20
One candidate inward-facing PCR primer pair, written to , isThe forward PCR primer binds the intronic flank immediately upstream of exon 5; the reverse PCR primer is the reverse complement of a downstream exon-5 segment. Their amplicon includes the repeat while both binding sites lie outside it. Using the displayed reference sequence gives an approximately -base-pair product; an indel changes the intervening length and sequence. The primers have matched simple Wallace-rule estimates near . These are candidates from the supplied sequence: confirm genomic specificity, absence of variable primer-binding sites, secondary structures and actual reaction conditions before use. Sequence from a unique flank toward the repeat and verify a single specific product.
New to topics? Read the docs here!