PCR primer

A PCR primer is a short DNA strand that supplies a paired

3^{'}

end for a DNA polymerase. Its nucleotide sequence determines where base pairing can initiate copying. Matching a PCR primer at one site is insufficient: both PCR primers must support opposed extension across a copyable interval for sustained selective PCR amplification.

Table of contents
- Primer melting temperature PCR primer

Primer melting temperature ( $T_{m}$ )

 0  0

The temperature at which a defined primer duplex is half dissociated under specified conditions. Sequence, salt and concentration matter; a simple composition rule is an approximation rather than a complete assay design.